NIH/3T3-CAS9 cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

NIH/3T3-CAS9 Order

Catalog#

YC-A013-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

NIH/3T3-CAS9

Morphology

Fibroblast-like, adherent

Passage ratio

1:3~1:5

Species

Mouse

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the NIH/3T3 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NIH/3T3-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
NIH/3T3-CAS9	Cas9	16.91583252	3.60078049
NIH/3T3-CAS9	h-actin-B	13.31505203	3.60078049
NIH/3T3	Cas9	33.85106277	20.10673046
NIH/3T3	h-actin-B	13.74433231	20.10673046

Cutting Efficiency Validation

NIH/3T3-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the NIH/3T3-CAS9 stable cell pool which is electroporated by Kras gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:NIH/3T3			STR Info (Cell bank) Cell Line:NIH-3T3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
4-2	233.16 【19.3】	237.36 【20.3】		19.3	20.3
5-5	335.38 【14】	339.45 【15】		14.0	15.0
6-4	290.24 【15.3】			15.3
6-7	334.14 【12】			12.0
9-2	220.72 【15】	224.74 【16】		15.0	16.0
12-1	241.32 【20】			20.0
15-3	192.53 【20.3】			20.3
18-3	155.35 【17】	163.49 【19】		17.0	19.0
X-1	399.83 【25】			25.0
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

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